Journal: Journal of Extracellular Vesicles
Article Title: Novel method for collecting hippocampal interstitial fluid extracellular vesicles (EV ISF ) reveals sex‐dependent changes in microglial EV proteome in response to Aβ pathology
doi: 10.1002/jev2.12398
Figure Lengend Snippet: Protein concentration increases with age and pathology while protein diversity decreases in EV ISF . (a) EV ISF protein concentration increased with age in both the WT ( p = 0.0006) and APP/PS1 mice ( p < 0.0001). (b) The number of unique protein IDs in EV ISF decreased with genotype ( p = 0.0020), age ( p = 0.0054), and pathology ( p = 0.0113) compared to 3‐month WT. (c) Venn Diagram reveals unique and shared EV ISF proteins among all four groups: 3‐month WT, 9‐month WT, 3‐month APP/PS1, and 9‐month APP/PS1. (d) Venn diagrams of proteins unique to and shared between 3‐month WT versus 3‐month APP/PS1, 3‐month WT versus 9‐month WT, 3‐month APP/PS1 versus 9‐month APP/PS1, 3‐month WT versus 9‐month APP/PS1, and 9‐month WT versus 9‐month APP/PS1. Protein diversity decreased with genotype, age, and pathology. (E) DAVID analyses revealed changes in the number of biological processes within groups with age, genotype and pathology. One‐way ANOVA, multiple comparisons Tukey's tests p < 0.05*, p < 0.01**, p < 0.001***, p < 0.0001****. N = 7–10 per group.
Article Snippet: Venn diagram comparing the EV ISF proteins from the four mouse groups was created using R Studio R package version 1.7.1.R “VennDiagram: Generate High–Resolution Venn and Euler Plots” by Hanbo Chen (2021) https://CRAN.R‐project.org/package = VennDiagram.
Techniques: Protein Concentration